Keeping the view in focus
A key component of the new setup is a spatial light modulator, which is used to manipulate the direction of the light and enable remote focusing. The spatial light modulator compensates the optical transfer function and images onto the fiber bundle. The back-reflected light from the fiber bundle is captured on the camera and superposed with a reference wave to measure the light’s phase.
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The position of the virtual guide star determines the instrument’s focus, with a minimal focus diameter of approximately one micron. The researchers used an adaptive lens and a 2D galvometer mirror to shift the focus and enable scanning at different depths.
Demonstrating 3D imaging
The team tested their device by using it to image a 3D specimen under a 140-micron thick cover slip. Scanning the image plane in 13 steps over 400 microns with an image rate of 4 cycles per second, the device successfully imaged particles at the top and bottom of the 3D specimen. However, its focus deteriorated as the galvometer mirror’s angle increased. The researchers suggest future work could address this limitation. In addition, using a galvometer scanner with a higher frame rate could allow faster image acquisition.
“The novel approach enables both real-time calibration and imaging with minimal invasiveness, important for in-situ 3D imaging, lab-on-a-chip-based mechanical cell manipulation, deep tissue in vivo optogenetics, and key-hole technical inspections,” said Czarske.
“Fast 3D Imaging with Lensless Holographic Endoscopy Employing Coherent Fiber Bundles,” by Juergen W. Czarske, Elias Scharf, and Robert Kuschmierz, will be presented Monday, 16 September 2019, at 11:15 a.m. EDT in Room Washington 6 of the Marriott Wardman Park hotel in Washington, D.C.
Award at Frontiers in Optics + Laser Science
Juergen W. Czarske will receive the 2019 Joseph Fraunhofer Award/Robert M. Burley Prize at FiO + LS for achievements in optical engineering. He is honored for seminal contributions to the field of digital interferometric and holographic sensors. Czarske is also a Fellow of OSA, EOS, SPIE, an elected member of Saxon Academy of Sciences and Scientific Society for Laser Technology. He has received the Berthold Leibinger Innovation Prize, the Reinhart Koselleck Project of German Research Foundation, the Measurement Technique Award of AHMT, and many other honors.
About Frontiers in Optics
Frontiers in Optics is The Optical Society’s (OSA) Annual Meeting and held together with Laser Science, a meeting sponsored by the American Physical Society’s Division of Laser Science (DLS). The two meetings unite the OSA and APS communities for five days of quality, cutting-edge presentations, in-demand invited speakers and a variety of special events spanning a broad range of topics in optics and photonics—the science of light—across the disciplines of physics, biology and chemistry. The exhibit floor will feature leading optics companies, technology products and programs.