Here is a Zeiss Axiovert 200 Fluorescence Phase Contrast Inverted Microscope Pred AXIO Observer
Setup for Brightfield with Phase Contrast and Fluorescence
This Zeiss Axiovert 200 Fluorescence Phase Contrast Inverted Microscope is in good working order with all aspects tested and working fully.
Microscope and optics have been professionally cleaned by our technicians under a class 10,000 clean room hood.
Please see the exact video and photos below.
Testing we performed:
The Zeiss Axiovert 200 Fluorescence Phase Contrast Inverted Microscopepassed all tests and was found to be fully functional:
Tested halogen lamp and source: PASSED
Tested mercury lamp and source: PASSED
Tested mechanical XY stage to limits: PASSED
Tested Z-axis focal adjustment: PASSED
Tested port splitter: PASSED
Tested objectives for clarity: PASSED
Tested eyepieces for clarity: PASSED
Tested connection to camera: PASSED
Tested live feed quality: PASSED
Tested all filters and prisms: PASSED
Tested motorized light control: PASSED
Tested both light paths: PASSED
During testing this Zeiss Axiovert 200 Fluorescence Phase Contrast Inverted Microscopewas found to have no issues.
A clear image was produced through the eyepieces as well as through the camera port.
All of the mechanical and Motorized components moved freely and the test sample was able to be focused without excessive effort.
System consists of:
Zeiss Axiovert 200 Inverted Microscope
(2) W-PI 10x/23 Eyepieces
Prisms / Condenser:
Mechanical Condenser with 0.55 Lens
Prisms: Ph1, Ph2
Axiovert Transmitted Illumination Tower
Built-in Fluorescence Light Path
Fluorescence Filter Cubes:
5-position Mechanical Turret
DAPI EX:340/380nm DM:400nm EM: 435/485nm
GFP BP EX:480/40nm DM:505nm EM: 535/50nm
TEXAS RED: EX: 540/580nm DM: 585nm EM: 630/75
HAL100 12V 100W Halogen Lamp with built-in power supply
EBQ 100W Fluorescence power supply with Zeiss 100w lamp housing
10x/0.30 Plan-NEOFLUAR Ph1
40x/0.75 Plan-NEOFLUAR Ph2
Photo / Port Splits:
100% Eye or 80% camera 20% Eye
AmScope MU300 3MP CMOS Digital Color Camera
Computer and Software:
Dell Latitude i5 Laptop running Windows 7 Pro w/ AmScope software
Communication and power cables.
Configuration and Setup:
Illumination: Brightfield with Phase Contrast and Fluorescence
Objectives: 5x/0.15 Epiplan-NEOFLUAR, 10x/0.30 Plan-NEOFLUAR Ph1, 40x/0.75 Plan-NEOFLUAR Ph2
Light Sources: 12V 100W Halogen Lamp, 100W Mercury Lamp
Stage Type: Mechanical XY
The Zeiss Axiovert 200 Fluorescence Phase Contrast Inverted Microscopeis currently set up for Brightfield viewing with Fluorescence and Phase Contrast studies.
The AmScope CMOS camera is capable of taking detailed digital images. The system includes a Dell Latitude i5 laptop to control AmScope software.
With included 5x, 10x and 40x objectives and 10x eypieces the total magnification of the system is 400x.
This video begins with a full front view of the Zeiss Axiovert 200 Fluorescence Phase Contrast Inverted Microscopepowered up and running.
The camera scans from one side to the other for a closer look. Next, we see the AmScope live feed and a demonstration with the manual focus and light intensity.
Next, fluorescence filters are moved into the light path showing illumination.
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