Here is a Zeiss Axioplan 2 Fluorescence Phase Contrast Microscope Pred Axio Scope.A1
Setup for Brightfield with Phase Contrast and Fluorescence
This Zeiss Axioplan 2 Fluorescence Phase Contrast Microscope is in good working order with all aspects tested and fully working.
Microscope optics have been cleaned professionally by our technician under a class 10,000 clean room hood.
Please see the exact video and photos below.
Testing we performed:
The Zeiss Axioplan 2 Fluorescence Phase Contrast Microscope passed all tests and was found to be fully functional:
Tested halogen lamp and source: PASSED
Tested mercury lamp and source: PASSED
Tested transmission halogen light path: PASSED
Tested reflective fluorescence light path: PASSED
Tested objective for clarity: PASSED
Tested port splitter to split between camera and eyepieces: PASSED
Tested condenser and prisms: PASSED
Tested motorized stage to limits: PASSED
Tested motorized Z-axis: PASSED
Tested reflector slider: PASSED
Tested connection from camera to laptop: PASSED
Tested control of all motorized axes via computer: PASSED
Tested live feed for clarity: PASSED
Tested all filter slides: PASSED
Tested all switches and adjustment knobs to be working: PASSED
During testing and cleaning this Zeiss Axioplan 2 Fluorescence Phase Contrast Microscope was found to have no issues.
A clear image was produced through the eyepieces as well as through the camera port.
All of the mechanical components moved freely and the test sample was able to be focused without excessive effort.
System consists of:
Zeiss Axioplan 2 Upright Microscope
(2) PI 10x/25 Eyepieces
Prisms and Condensers:
Condenser Lens 0.9
7-position Mechanical Condenser
Transmission halogen light path
Reflection fluorescence light path
Fluorescence Filter Cubes:
R&B PHY (Phycoerythrin/mOrange/mKO) - EX 546/10 BS 560 EM 585/40
GFP (Green) - Ex 470/40 BS 495 EM 525/50
HAL12V 100W Halogen Lamp with built-in power supply
HBO 100 Mercury Lamp with 100W ebq100 power supply
75 x 50mm Motorized Stage
LEP MAC5000 XYZ Motorized Control
20x/0.30 D A-Plan Ph1
Trinocular Eye 100% or 100% Camera 50/50% split
AmScope MU300 CMOS 3.1MP Digital Color Camera
Computer and Software:
Dell Latitude i5 running Windows 7 Pro with AmScope and MicroManager software
Communication and power cables.
Configuration and Setup:
Illumination Type: Brightfield w/ Phase Contrast, Fluorescence
Objectives: 4x/0.10 ACHROPLAN, 10x/0.30 Plan-NEOFLUAR, 20x/0.30 D A-Plan Ph1
Light Sources: HAL100 12V 100W Halogen Lamp, HBO100 Mercury Lamp
Stage Type: Motorized XY
This Zeiss Axioplan 2 Fluorescence Phase Contrast Microscope is currently set up for Brightfeild and Phase Contrast Transmitted light and Fluorescence Reflected light.
The AmScope MU300 CMOS digital camera is capable of taking detailed digital images.
This Zeiss system includes a Dell i5 Laptop running Windows 7 with AmScope software and MicroManager software to control the motorized XYZ axes.
The 10x eyepieces combined with the 4x, 10x and 20x objectives give maximum magnification 200x.
The video starts off with a full view of the Zeiss Axioplan 2 Fluorescence Phase Contrast Microscope.
The camera pans into the live feed of the sample then around the entire Zeiss Axioplan 2 Fluorescence Phase Contrast Microscope bringing into focus all included aspects and components.
The XYZ joystick is then used to move around the sample on the lie feed. The objective is rotated and the motorized Z-axis is used to re-focus the sample.
The halogen lamp is turned off and the and the fluorescence filter are moved into the reflective path.
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