For Sale Agilent 5300 Fragment Analyzer
Pre-owned instrument
• Fully Functional
• Current Model
• Serviced & in Stock
• Good cosmetic condition
• Warranty
• An extended warranty option and training may be available.
Made in 2019
Here is what you will receive:
• Agilent 5300 Fragment Analyzer
• Capillary Array
• (4) Marker/sample PCR microplate (semi or non-skirted) holders
• (2) Deep-well microplate holders
• (2) 250ml bottle
• 50ml bottle
• 500ml waste bottle
• Computer with Fragment Analyzer ProSize data analysis software
• Fragment Analyzer controller software
• Syringe for valve flush
• Communication and power cables
Please note: The Capillary Array is a consumable and has a one-year or 1000-injection lifespan. A new one will need to be purchased from Agilent for around $3540.
Agilent 5300 Fragment Analyzer Configuration:
• Instrument Applications: NGS libraries, cell-free DNA, genomic DNA, large fragment DNA, PCR fragments, total RNA and IVT RNA
• Analysis Method: fluorescence-based capillary electrophoresis using intercalating dye
• Capillary Array: compatible with 48 and 96 capillary arrays
• Minimum Sample Volume: 25 uL of liquid for injection; as little as 2.5 uL of actual sample required
• Sample Capacity: 3 sample trays, each holding 1x 96 well PCR microplate
• Compatible Microplates: 96 well semi or non-skirted PCR plates for markers and samples, 96 deep-well plates for buffer and waste
• Number of Samples per Run: 48 or 96 (Depending on the installed Array)
• Variable Throughput: up to 240 samples, medium throughput system
• High Sensitivity: sensitivities down to 5 pg/uL for a single fragment, cycle time or PCR reagent
• Resolution and Sizing Accuracy: resolve small differences between fragments, as low as 3 bp for fragments under 300 bp size
Customization/Options: n/a
Testing performed by our in-house service team:
• We checked the power supply to ensure it provides stable output.
• We test communication with the software to verify seamless interaction and data exchange.
• We test the initialization and homing processes to guarantee accurate and reliable setup.
• We check the pump operation to ensure consistent and reliable fluid movement.
• We test the plate movement to validate precise and smooth sample handling.
• We thoroughly test the prime function of all fluids to ensure the system is clean and functions as designed.
• We test the pressurization system from minimum to maximum levels to ensure stability and controlled pressure conditions.
• We test the high voltage system from minimum to maximum levels to verify consistent and safe operation.
• We performed an Optical Alignment on a Capillary Array, verifying the camera and illumination were operating fully.
Instrument Overview:
• Agilent 5300 Fragment Analyzer utilizes capillary electrophoresis for high-resolution nucleic acid analysis.
• Advanced algorithms enable precise sizing and quantification of DNA and RNA fragments.
• Quality control capabilities include assessing genomics workflows with exceptional accuracy.
• The instrument streamlines the evaluation of PCR and NGS library preparations.
• Robust technology ensures not only accurate quantification but also comprehensive integrity assessment for diverse samples in molecular biology research.
Instrument Application:
Agilent 5300 Fragment Analyzer is commonly used in a variety of assay and application examples, including:
• Nucleic acid fragment analysis
• Quality control in genomics workflows
• Size determination of DNA and RNA fragments
• Assessment of PCR and NGS library preparations
• Quantification and qualification of sample integrity
• High-throughput DNA and RNA analysis for research and diagnostics
The key features of Agilent 5300 Fragment Analyzer:
• High-precision DNA/RNA analysis
• High-resolution separation
• Medium- to high-throughput separation
• User-friendly interface
• Reduced sample analysis time
• Broad assay portfolio
• Controlled sample handling
• Seamless switching between applications
Why should you buy from us?
Our Advantage
• We have experience servicing and repairing these instruments.
• This instrument goes through all the described service steps before shipping.
• We offer detailed photos and videos of the instruments you buy.
• We are an approved pre-owned equipment vendor to many large US, Canadian, and EU Universities.
• We are an approved pre-owned equipment vendor to many Fortune 500 US and International Biopharma companies.
• Many upgrade and repair parts are in stock.
• Remote installation and instrument operation training are available.
• On-site installation may be available in the Boston Area (New England area)
• US or International shipping is no problem (see the shipping tab on how we pack and feel at ease)
Our Guarantee
• It will work!
• Warranty
• An extended warranty may be available.
• You get after-sales technical support.
• Smooth process from the initial quote to order fulfillment.
• Customer references are available.
*ME* Medium Crate
350#
PLEASE NOTE THAT THE QUESTIONS BELOW ARE ANSWERED IN MORE THOROUGH DETAIL ON OUR WEBSITE
Q: Can I view this instrument in person at your facility or do a live Zoom demo before it is shipped to me?
A: We can accommodate both in-person or Zoom demos.
Q: What about the warranty?
A: The instrument comes fully warranted for both parts and labor.
Q: Do you offer installation, service, etc?
A: We offer remote installation and training packages to familiarize you with this instrument.
Q: What should I watch out for when buying a used/pre-owned instrument from another lab?
A: Ensure that the instrument is in good working condition with no damage or wear that could affect its performance. Ask for details about testing steps performed, video demo, and test screenshots.
Q: Do you have more than one of these instruments on hand?
A: We generally carry multiple instruments on hand, along with a variety of spare parts.
Q: This procurement is for a University or College. Are purchase orders with NET terms accepted?
A: We accept purchase orders from all major US and Canadian universities.
Q: How fast will you ship?
A: We can ship within a few days to two weeks. Shipping time depends on the current demand for our instruments. Members of our sales team will be able to offer current delivery estimates.
Q: Will my instrument be insured during shipping?
A: Absolutely! We insure all our shipments as long as we are the shipper. If a customer ships on their account, they handle insurance.
Q: How do you pack? Have you previously packed and shipped the fragile laboratory instruments?
A: We have extensive experience shipping high-end and complex laboratory instruments in the US and Internationally.
Q: Do you ship Internationally?
A: So far, we have shipped to almost every continent and over 30 countries.
Q: How much will I save buying it pre-owned, and is it worth it?
A: When compared to buying a new instrument, you will save 50% or more and have plenty of funds left over for an additional instrument or two.
TO VIEW ADDITIONAL PHOTOS, VIDEOS AND VERY DETAILED INFORMATION SUCH AS TEST STEPS PERFORMED, CONFIGURATION, ETC., PLEASE VISIT OUR WEB SITE. TO GET THERE PLEASE CLICK ON “WEBSTORE”; TO YOUR RIGHT AND YOU WILL SEE LINK TO OUR SITE IN THE MIDDLE OF THE SCREEN. THEN USE LAST 5 DIGITS OF MPN NUMBER IN THE SEARCH FIELD TO LOCATE YOUR ITEM.
DO NOT HESITATE TO CALL US OR EMAIL US WITH ANY QUESTIONS YOU MIGHT HAVE.
ALL OF OUR ITEMS ARE AVAILABLE FOR INSPECTION AT OUR FACILITY.
Return Policy
Items are sold as-is with no returns or refunds available unless explicitly stated.
